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KMID : 0616620030090010021
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Journal of Soonchunhyang Medical College
2003 Volume.9 No. 1 p.21 ~ p.27
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Expression of p53 and Ki-67 in squamous cell carcinoma, actinic keratosis and Bowen’s disease
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Jang Young-Woo
Yang Seoung-Ha
Kim Eui-Han
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Abstract
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Background: The function of p53 protein was known to regulate damaged cell proliferation in S phase, so damaged cell proliferation was not occurred by apoptosis. Ki-67 nuclear antigen has been used as a marker to define growth fraction in both benign and malignant tumors. The Ki-67 nuclear antigen was expressed throughout the cell cycle except G0 and early G1 phase. The role of p53 and Ki-67 in oncogenesis, tumor progression and prognosis were important. The authors analyzed immunohistochemical expression of p53 and Ki-67 in squamous cell carcinoma, actinic keratosis and Bowen’s disease.
Method: Seventeen cases of squamous cell carcinoma, fifteen cases of actinic keratosi and seven cases of Bowen’s disease were immunohistochemically stained with p53 and Ki-67 monoclonal antibodies.
Results: 1. p53 protein were expressed mainly in basal layer in well differentiated squamous cell carcinoma and diffusely expressed in poorly differentiated squamous cell carcinoma. In actinic keratosis, the expression pattern of p53 was similar to well differentiated squamous cell carcinoma, but the intensity was lower. 2. In Bowen’s disease, the p53 protein was more diffusely and strongly expressed than squamous cell carcinoma. 3. The ki-67 labelling index in squamous cell carcinoma was below 20% regardless of differentiation. In actinic keratosis, the labelling index was lower. 4. The Ki-67 labelling index in Bowen’s disease was higher than squamous cell carcinoma and weakly expressed in basal layer, especially adjoining dermis. 5. In mitotic cells, the p53 protein was not expressed, but Ki-67 nuclear antige was strongly expressed. Above results suggested that Bowen’s disease is almostly composed of immature cell while squamous cell carcinoma was composed of the mature and immature cells. In spite of higher labelling index in Bowen’s disease, the growth rate is really faster in squamous cell carcinoma. The authors suggested the reason that the number of the mitotic cells in Bowen’s disease were fewer than that of squamous cell carcinoma.
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